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Evidence Guide: AHCSDT408 - Perform an electrophoresis test on a seed sample

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

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AHCSDT408 - Perform an electrophoresis test on a seed sample

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare seed extraction and gel

  1. Prepare working sample from submitted sample
  2. Identify seed to be tested and complete records
  3. Cut or crush single seeds and transfer to centrifuge tubes
  4. Add extraction solution and mix with crushed seeds and allow to stand for prescribed period
  5. Centrifuge tubes and collect and store supernatants
  6. Prepare gel mixture and chemicals and use to achieve required level of polymerisation
Prepare working sample from submitted sample

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify seed to be tested and complete records

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut or crush single seeds and transfer to centrifuge tubes

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Add extraction solution and mix with crushed seeds and allow to stand for prescribed period

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Centrifuge tubes and collect and store supernatants

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare gel mixture and chemicals and use to achieve required level of polymerisation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Carry out electrophoresis

  1. Place buffer solution in tanks
  2. Load samples into wells and place gel in tanks
  3. Carry out electrophoresis at the prescribed voltage and duration
  4. Maintain temperature as required throughout the process
Place buffer solution in tanks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Load samples into wells and place gel in tanks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Carry out electrophoresis at the prescribed voltage and duration

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain temperature as required throughout the process

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Fix and stain sample

  1. Remove gel from tank and treat with chemicals as prescribed
  2. Wash gel and treat to enhance stain
  3. Examine and/or photograph stain
Remove gel from tank and treat with chemicals as prescribed

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Wash gel and treat to enhance stain

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine and/or photograph stain

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Evaluate stain

  1. Compare banding pattern with that of an authentic variety or that of a known control sample
  2. Provide records and reports
  3. Determine if obvious procedure or equipment problems have led to atypical data or results
Compare banding pattern with that of an authentic variety or that of a known control sample

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Provide records and reports

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Determine if obvious procedure or equipment problems have led to atypical data or results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Use established safe work practices and personal protective equipment
  2. Minimise the generation of wastes and environmental impacts
  3. Ensure the safe collection of laboratory and hazardous waste for subsequent disposal
  4. Care for and store equipment and reagents
Use established safe work practices and personal protective equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Minimise the generation of wastes and environmental impacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Care for and store equipment and reagents

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain laboratory records

  1. Enter approved data into laboratory information management system
  2. Maintain confidentiality and security of enterprise information and laboratory data
Enter approved data into laboratory information management system

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain confidentiality and security of enterprise information and laboratory data

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare seed extraction and gel

1.1 Prepare working sample from submitted sample

1.2 Identify seed to be tested and complete records

1.3 Cut or crush single seeds and transfer to centrifuge tubes

1.4 Add extraction solution and mix with crushed seeds and allow to stand for prescribed period

1.5 Centrifuge tubes and collect and store supernatants

1.6 Prepare gel mixture and chemicals and use to achieve required level of polymerisation

2. Carry out electrophoresis

2.1 Place buffer solution in tanks

2.2 Load samples into wells and place gel in tanks

2.3 Carry out electrophoresis at the prescribed voltage and duration

2.4 Maintain temperature as required throughout the process

3. Fix and stain sample

3.1 Remove gel from tank and treat with chemicals as prescribed

3.2 Wash gel and treat to enhance stain

3.3 Examine and/or photograph stain

4. Evaluate stain

4.1 Compare banding pattern with that of an authentic variety or that of a known control sample

4.2 Provide records and reports

4.3 Determine if obvious procedure or equipment problems have led to atypical data or results

5. Maintain a safe work environment

5.1 Use established safe work practices and personal protective equipment

5.2 Minimise the generation of wastes and environmental impacts

5.3 Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

5.4 Care for and store equipment and reagents

6. Maintain laboratory records

6.1 Enter approved data into laboratory information management system

6.2 Maintain confidentiality and security of enterprise information and laboratory data

Required Skills and Knowledge

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare seed extraction and gel

1.1 Prepare working sample from submitted sample

1.2 Identify seed to be tested and complete records

1.3 Cut or crush single seeds and transfer to centrifuge tubes

1.4 Add extraction solution and mix with crushed seeds and allow to stand for prescribed period

1.5 Centrifuge tubes and collect and store supernatants

1.6 Prepare gel mixture and chemicals and use to achieve required level of polymerisation

2. Carry out electrophoresis

2.1 Place buffer solution in tanks

2.2 Load samples into wells and place gel in tanks

2.3 Carry out electrophoresis at the prescribed voltage and duration

2.4 Maintain temperature as required throughout the process

3. Fix and stain sample

3.1 Remove gel from tank and treat with chemicals as prescribed

3.2 Wash gel and treat to enhance stain

3.3 Examine and/or photograph stain

4. Evaluate stain

4.1 Compare banding pattern with that of an authentic variety or that of a known control sample

4.2 Provide records and reports

4.3 Determine if obvious procedure or equipment problems have led to atypical data or results

5. Maintain a safe work environment

5.1 Use established safe work practices and personal protective equipment

5.2 Minimise the generation of wastes and environmental impacts

5.3 Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

5.4 Care for and store equipment and reagents

6. Maintain laboratory records

6.1 Enter approved data into laboratory information management system

6.2 Maintain confidentiality and security of enterprise information and laboratory data

The candidate must be assessed on their ability to integrate and apply the performance requirements of this unit in a workplace setting. Performance must be demonstrated consistently over time and in a suitable range of contexts.

The candidate must provide evidence that they can:

extract seed and prepare gel

centrifuge seed and gel mixes

interpret stain bands and patterns

test seed by separating proteins of varying size and/or electric charge

enter data into laboratory information management system

maintain confidentiality and security of information and data

The candidate must demonstrate knowledge of:

enterprise standard operating procedures (SOPs)

materials and apparatus used in electrophoresis testing

principles of electrophoresis

range of electrophoresis testing procedures

relevant health, safety and environment requirements

seed biology

the role of the International Seed Testing Association (ISTA) and the ISTA Rules for Seed Testing

using stain patterns and ‘fingerprints’ to determine seed variety

Range Statement